Secretory vesicle trafficking and recycling
We study the transport, docking, fusion and retrieval of two type secretory vesicles: small translucent neurotransmitter vesicles in pre-synapse of neurons and neuropeptide-containing, large dense-core vesicles in neurons and neuro-endocrine cells. The focus lies on the interactions between these secretory vesicles and the cytoskeleton as well as protein/lipid interactions in genetic model systems for disease.
Our research group aims to uncover the molecular mechanisms involved in the membrane trafficking pathways, utilized by secretory vesicles at nanometer resolution.
We are embedded in the VU/VUmc Electron Microscopy Facility. There, we utilize advanced electron microscopy techniques to study these membrane transport steps in nanoscale resolution. This is combined with a wide variety of fluorescence microscopy techniques to investigate dynamic processes in real time, both in living neurons and neuroendocrine cells.
- immuno-electron microscopy
- cryo-electron microscopy
- electron tomography
- correlative light and electron microscopy
- live cell imaging (TIRF, pHluorin)
- superresolution light microscopy (STORM)
- confocal microscopy
- protein purification
van Weering, J.R.T., Sessions, R.B., Traer, C.J., Kloer D.P., Bhatia V.K., Stamou D., Hurley, J.H. and Cullen P.J. Molecular basis for SNX-BAR-mediated assembly of distinct endosomal sorting tubules. EMBO J (2012), vol 31 (23) pp. 4466-80
van Weering, J.R.T., Verkade, P., Cullen, P.J. SNX-BAR-mediated endosome tubulation is coordinated with endosome maturation. Traffic (2012), vol. 13 (1) pp. 94-107
van Weering, J.R.T.*, Schonn, J.S.*, Mohrmann, R.*, Schlüter, O.M., Südhof, T.C., de Wit, H., Verhage, M., and Sørensen, J.B. Rab3 proteins involved in vesicle biogenesis and priming in embryonic mouse chromaffin cells. Traffic (2010) vol. 11 (11) pp. 1415-28